Studying of the interleukin-36γ expression level in the skin of patients with plaque psoriasis

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  • Authors: Pashkin A.Y.1, Zhukov A.S.2, Khairutdinov V.R.2, Belousova I.E.2, Samtsov A.V.2, Garabagiou A.V.3
  • Affiliations:
    1. The 3d Military Hospital, National Guard Forces
    2. S. M. Kirov Military Medical Academy, Ministry of Defence of the Russian Federation
    3. Saint Petersburg State Institute of Technology (Technical University)
  • Issue: Vol 95, No 4 (2019)
  • Pages: 31-39
  • Section: SCIENTTIFIC RESEARCHES
  • URL: https://www.vestnikdv.ru/jour/article/view/502
  • DOI: https://doi.org/10.25208/0042-4609-2019-95-4-31-39

Abstract


Currently, it has been established that the cytokines of the IL-36 family occupy a significant place in the initiation and regulation of the inflammatory process in psoriasis.

Objective: studying the expression level of IL-36γ cytokines in the skin of patients with plaque psoriasis.

Material and methods. Skin biopsy specimens of 31 patients with plaque psoriasis were studied. The comparison group consisted of 20 biopsy samples of the skin of patients with lichen simplex, discoid eczema, lichen planus, mycosis fungoides (plaque stage). As a control group studied the skin bioptates of 10 healthy people. An immunohistochemical study of the skin was carried out using anti-IL-36γ antibodies.

Results. An increase in the relative expression area of IL-36γ in the affected skin of patients with plaque psoriasis (7.4 %) was found, compared with the unaffected areas (0.10 %) and the control group (0 %). The expression of IL-36γ in the skin of patients with psoriasis in the progressive period (8.85 %) was 1.42 times higher than in the stationary period of the disease (6.2 %). A strong direct relationship was revealed (r = 0.71) between the level of IL-36γ expression in the affected skin and the value of the PASI index, a moderate direct relationship between the level of IL-36γ expression and epidermal thickness (r = 0.34). In the affected skin of psoriasis patients, expressed expression of IL-36γ was observed in the upper layers of the epidermis, patients of the comparison group (discoid eczema, lichen simplex, lichen planus, mycosis fungoides) were weak or moderate, in the unaffected areas of the skin of patients with psoriasis and healthy people — weak or absent.

Findings. It was found that the expression of IL-36γ in the skin of patients with plaque psoriasis is significantly higher than with other skin diseases. The data obtained allow us to consider this cytokine as a possible diagnostic marker and use it in the differential diagnosis. 


About the authors

A. Yu. Pashkin

The 3d Military Hospital, National Guard Forces

Email: alek-pashkin@yandex.ru

Russian Federation

Dermatovenerologist, Head of the Department,

Tsimbalina str., 36. Saint Petersburg, 192171

A. S. Zhukov

S. M. Kirov Military Medical Academy, Ministry of Defence of the Russian Federation

Email: doctor-vma@mail.ru

Russian Federation

Cand. Sci. (Med.), Doctoral Student, Department of Skin and Sexually Transmitted Diseases,

Akademika Lebedeva str., 6, Saint Petersburg, 194044

V. R. Khairutdinov

S. M. Kirov Military Medical Academy, Ministry of Defence of the Russian Federation

Author for correspondence.
Email: haric03@list.ru

Russian Federation

Dr. Sci. (Med.), Assoc. Prof., Department of Skin and Sexually Transmitted Diseases,

Akademika Lebedeva str., 6, Saint Petersburg, 194044

I. E. Belousova

S. M. Kirov Military Medical Academy, Ministry of Defence of the Russian Federation

Email: irena.belousova@mail.ru

Russian Federation

Dr. Sci. (Med.), Assoc. Prof., Prof., Department of Skin and Sexually Transmitted Diseases,

Akademika Lebedeva str., 6, Saint Petersburg, 194044

A. V. Samtsov

S. M. Kirov Military Medical Academy, Ministry of Defence of the Russian Federation

Email: avsamtsov@mail.ru

Russian Federation

Dr. Sci. (Med.), Prof., Head of the Department of Skin and Sexually Transmitted Diseases, 

Akademika Lebedeva str., 6, Saint Petersburg, 194044

A. V. Garabagiou

Saint Petersburg State Institute of Technology (Technical University)

Email: gar-54@mail.ru

Russian Federation

Dr. Sci. (Chemistry), Prof., Vice-Rector for Scientific Work,

Moskovskiy prospect, 26, Saint Petersburg, 190013

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