Main level of cytokines in patients with psoriasis

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Abstract


The aim of the study was to determine the level of cytokines in the serum of patients with psoriatic arthritis (PsA) and psoriasis vulgaris (Ps) and to identify key immune markers associated with clinical manifestations of psoriasis. Materials and methods. The study involved 52 patients with psoriatic arthritis (PsA) and 20 patients with psoriasis vulgaris (PS) by multiplex analysis of proteins using Bio-Plex device and commercial test kits 23-Plex and 27-Plex, 8-Plex. Assessment of the severity and prevalence of psoriatic skin lesions in patients was conducted by index PASI (Psoriasis Area and Severity Index). Indicators of acute-phase activity (ESR, CRP), CEC were defined in all patients. the comparison group consisted of patients with a diagnosis of patchy scleroderma (10 patients) and patients with atopic dermatitis (10 patients). 13 healthy individuals were examined as a control group. Main results. Significant elevation of cytokines: IL-2, IL-6, GM-CSF, IFN-y and TNF-a was found in the serum of patients with PS compared with the group of apparently healthy individuals, and GM-CSF, IFN-y compared with patients with atopic dermatitis. It was determined that the detected levels of Il-В in serum has a direct correlation with the prevalence and severity of PS. Inverse correlation between ESR and the level of IL-8, GM-CSF and the CEC, IFN-y and the CEC and the direct correlation between the level of TNF-a and CRP in serum were shown. Conclusion. The obtained data indicate shifts in the system of pro-inflammatory and anti-inflammatory cytokines in psoriasis, which can be considered as a manifestation of endogenous homeostatic mechanisms designed to limit the intensity of the inflammatory process. Immune markers associated with clinical manifestations of psoriasis, in particular, the prevalence and severity of PS (index PASI), indicators of acute-phase activity (ESR, CRP), were identified.

  1. Shegay M.M., Keshileva Z.B., Akishbayeva G.A. The role of some cytokines in the development of psoriasis. Vestn dermatol venerol 1998; 5 (7): 13-21. [Шегай М.М., Кешилева З.Б., Акышбаева Г.А. Роль некоторых цитокинов в развитии псориаза. Вестн дерматол венерол 1998; 5 (7): 13-21.]
  2. Pietrzak A.T., Zalewska A., Chodorowska G., Krasowska D., Michalak-Stoma A., Nockowski P., Osemlak P., Paszkowski T., Rolinski J.M. Cytokines and anticytokines in psoriasis. Clin Chim Acta 2008; 394 (1-2): 7-21.
  3. Beltyukova A.S., Sysoev K.A., Khobeysh M.M., Monakhov K.N., Sokolovsky E.V., Totolyan Areg A. Determination of 50 cytokines in serum of patients with psoriasis. Modern probl Dermatol Venereol, immunol and med cosmetol 2011; 2 (15): 9-15. [Бельтюкова А.С., Сысоев К.А., Хобейш М.М., Монахов К.Н., Соколовский Е.В., Тотолян Арег А. Определение уровня 50 цитокинов в сыворотке крови больных псориазом. Соврем пробл дермато-венер, иммунол и врач косметол 2011; 2 (15): 9-15.]
  4. Khobeysh M.M., Beltyukova A.S. Determination of potentially relevant cytokines in patients with severe psoriasis. Abstracts of scientific publications. VI St. Petersburg dermatological reading. 2012. p. 31. [
  5. Хобейш М.М., Бельтюкова А.С. Определение содержания потенциально значимых цитокинов у больных тяжелыми формами псориаза. Тезисы научных работ Vi Санкт-Петербургские дерматологические чтения, СПб, 2012. С. 31.]
  6. Schlaak J.F., Buslau M., Jochum W., Hermann E., Girndt M., Gallati H., Meyer zum Büschenfelde K.H., Fleischer B. T cells involved in psoriasis vulgaris belong to the Th1 subset. J Invest Dermatol 1994; 102 (2): 145-149.
  7. Nickoloff B.J., Karabin G.D., Barker J.N., Griffiths C.E., Sarma V., Mitra R.S., Elder J.T., Kunkel S.L., Dixit V.M. Localization of IL-8 and its inducer tumor necrosis factor-alpha in psoriasis. Am J Pathol 1991; 138 (1): 129-140.
  8. Beltyukova A.S., Sysoev K.A., Khobeysh M.M., Monakhov K.N., Ochelenko S.A., Ilina T.N., Shemerovsky T.G., Totolyan Areg A. Expression of FOXP3 in skin in psoriasis. Immunology 2009; 6: 361-364. [Бельтюкова А.С., Сысоев К.А., Хобейш М.М., Монахов К.Н., Очеленко С.А, Ильина Т.Н., Шемеровская Т.Г., Тотолян Арег А. Экспрессия FOXP3 в коже при псориазе. Иммунология 2009; (6): 361-364.]
  9. Hijnen D., Knol E.F., Gent Y.Y., Giovannone B., Beijn S.J., Kupper T.S., Bruijnzeel-Koomen C.A., Clark R.A. CD8 (+) T cells in the lesional skin of atopic dermatitis and psoriasis patients are an important source of IFN-y, IL-13, IL-17 and IL-22. J Invest Dermatol 2013; 133 (4): 973-979.
  10. Beltyukova A.S., Sysoev K.A., Ilina T.N., Shemerovsky T.G., Khobeysh M.M., Monakhov K.N., Areg A. Totolyan. MRNA expression of chemokines and chemokine receptors in the skin of patients with psoriasis. Medical Immunology 2008; 10 (4-5): 337-346. [Бельтюкова А.С., Сысоев К.А., Ильина Т.Н., Шемеровская Т.Г., Хобейш М.М., Монахов К.Н., Тотолян Арег А. Экспрессия мРНК хемокинов и хемокиновых рецепторов в коже больных с псориазом. Медицинская иммунология 2008; 10 (4-5): 337-346.]
  11. Gottlieb A.B. Infliximab for psoriasis. J Am Acad Dermatol 2003; 49 (2): 112-117.
  12. Zalewska A., G+owacka E., Wyczokowska J., Tchorzewski H., Narbutt J., Sysa-Jedrzejowska A. Interleukin 6 and 8 levels in plasma and fibroblast cultures in psoriasis. Mediators Inflamm 2006; 1: 817-867.
  13. Nickoloff B.J. The immunologic and genetic basis of psoriasis. Arch Dermatol 1999; 135: 1104-1110.
  14. Fierlbeck G., Rassner G., Muller C. Psoriasis induced at the injection site of recombinant interferon gamma. Results of immunohistologic investigations. Arch Dermatol 1990; 126: 351-355.
  15. Bonifati C., Ameglio F. Cytokines in psoriasis. Int J Dermatol 1999; 38: 241-251.
  16. Totolyan A.A., Freidlin I.S. Cells of the immune system. St. Petersburg: Nauka, 2000; I-II. 28-33. [Тотолян А.А., Фрейдлин И.С. Клетки иммунной системы. СПб: Наука 2000; I-II: 28-33.]
  17. Krinner E.M., Raum T., Petsch S., Bruckmaier S., Schuster I., Petersen L., Cierpka R., Abebe D., Molhoj M., Wolf A., Sorensen P., Locher M., Baeuerle P.A., Hepp J. A human monoclonal IgG1 potently neutralizing the pro-inflammatory cytokine GM-CSF. Mol Immunol 2007; 44 (5): 916-925.
  18. Kelly R., Marsden R.A., Bevan D. Exacerbation of psoriasis with GM-CSF therapy. Br J Dermatol 1993; 128: 468-469.

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