Using IgM methods in the diagnostics of syphilis

Cover Page

Abstract


The authors present the results of a questionnaire survey conducted in serological laboratories in 60 dermatovenerology enterprises in the subjects of the Russian Federation on the use of up-to-date methods for the determination of Class M antibodies for the diagnostics of early syphilis. It was revealed that treponemal specific Class M antibodies were determined in 86.67% of laboratories: the EIAIgM method was used in 86.67% of laboratories, IBIgM - in 18.33%, and PHTIgM - in 6.67% of laboratories only. The total number of IgM tests did not exceed 1.3% of the entire volume of immunochemical reactions conducted for the diagnostics of syphilis in laboratories in 2012; as for IgM methods, the use of EIAIgM prevailed - 97.27% while the frequency of using PHTIgM and IBIgM was 2.0 and 0.73%, respectively. In the authors’ opinion, such ratios were stipulated by the absence of production of kits made in Russia during the study period (in 2012) and allowed for use for methods such as PHTIgM and IBIgM while there was a wide choice of kits required for EIAIgM and laboratories had a long-term experience of using this method.

S. V. Rotanov

State Research Center of Dermatovenereology and Cosmetology, Ministry of Healthcare of the Russian Federation; The Russian National Research Medical University named after N.I. Pirogov (RNRMU)

Author for correspondence.
Email: rotanov@cnikvi.ru

Russian Federation

F. A. Ermatova

The Russian National Research Medical University named after N.I. Pirogov (RNRMU)

Email: noemail@neicon.ru

Russian Federation

  1. Federal law No. 323-FZ dated 21.11.2011 "On the basis of health protection in the Russian Federation". Collected legislation of the Russian Federation, 2011, No. 48, article 6724
  2. Order of the Ministry of health and social development of the Russian Federation No.543-n dated 15.05.2012 "On approval of the Regulations on the organization providing primary health care to the adult population
  3. Order of the Ministry of health of the Russian Federation No. 942-n dated 15.11.2012 "On approval of the Procedure of rendering of medical aid to the population in the profile "dermatovenerology" (registered No. 26302 in the Ministry of justice of Russia 21.12.2012). "Russian gaseta", spec. issue No. 78/1, 11.04.2013
  4. Luger A. Serological diagnosis of syphilis; current methods. In: Young H., McMillan A. (Eds.). Immunological diagnosis of sexually transmitted diseases. N-Y: Marcel Dekker 1998: 249-274.
  5. Egglestone S.I., Turner A.J.L. Serological diagnosis of syphilis. Commun Dis Public Health 2000; 3: 158-162.
  6. Young H. Guidelines for serological testing for syphilis. Sex Transm Inf 2000; 76: 403-405.
  7. Goh B.T., van Voorst Vader P.C. European Guideline for the management of syphilis. Int J of STD & AIDS 2001; 12 (Suppl. 3): 14-26.
  8. Serdjuckaja M.B., Ivanov A.M., Ovsyannikov F.A. Gupalova T.V., Hodosevich E.V. Method of increasing the sensitivity of detection of IgM antibodies in the diagnosis of congenital syphilis. Russian journal of immunology 2007; 9 (Suppl 1): 128-129.
  9. Krasnoselskikh T.V., Sokolovskiy E.F. Modern syphilis: epidemiological trends achievements in the study of Treponema pallidum. Probl modern dermatol, venereol and cosmetol 2010; 1: 84-87
  10. Larsen S.A. (Ed.), Pope V., Johnson R.E., Kennedy E.J. Manual of tests for syphilis. 9th ed. Washington: DC, American Health Association, 1998.
  11. Order of the Ministry of health of the Russian Federation No. 87 dated 26.03.2001 "On improving serological diagnosis of syphilis" (Annex 1 of "Setting the selection and diagnostic tests for syphilis."
  12. Lomonosov K.M. Actual interview. New in the diagnosis and treatment of syphilis. Rus j skin and venere dis 2002; 5: 56-60.
  13. Kingston M., French P., Goh B. UK National Guidelines of the Management of Syphilis 2008. Int J of STD & AIDS 2008 (Nov); 19: 729-740.
  14. Kitaeva N.V., Frigo N.V., Melechina L.E. Actual problems of syphilidology. Modern techniques for the diagnosis of syphilis infection. Vestn of dermatol venereal 2008; 5: 51-59.
  15. Frigo N.V., Rotanov S.V., Manukyan T.E., Katunin G.L., Suvorova A.A., Volkov I.A., Kitaeva N.V. Laboratory diagnosis of syphilis: yesterday, today and tomorrow. Vestn dermatol venereal 2012; 4: 16-23
  16. Lyakhov V.F., Borisenko K.K., Potekaev N.N. et al. Dynamics anti-treponemal immunoglobulinemia with early forms of syphilis. Vestn dermatol venereal 1990; 8: 38-42
  17. Sidorova E.V., Lyakhov V.F. The value of determination anti-treponemal IgM antibodies in the serodiagnosing of syphilis. STD 1995; 4: 11-14.
  18. Tkachev V.K., Vyatkin T.G. ELISA test for diagnosis of syphilis: information guide. 3rd ed., 2005. URL: http://www.vector-best.ru/brosh/9607.htm
  19. Resources and activity dermatological medical organizations profile. The incidence of sexually transmitted diseases, infectious skin diseases and diseases of the skin for 2011-2012

Views

Abstract - 62

PDF (Russian) - 26

Refbacks

  • There are currently no refbacks.

Copyright (c)



This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies