Development of an experimental model of pemphigus vulgaris in laboratory animals

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  • Authors: Kubanov A.A.1, Karamova A.E.1, Rog K.V.1, Abramova T.V.2, Smolyannikova V.A.3, Murashev A.N.4, Bondarenko D.A.4
  • Affiliations:
    1. State Research Center of Dermatovenereology and Cosmetology, Ministry of Healthcare of the Russian Federation
    2. Russian Medical Academy of Postgraduate Studies, Ministry of Health of the Russian Federation
    3. I. M. Sechenov First Moscow State Medical University
    4. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences
  • Issue: Vol 91, No 4 (2015)
  • Pages: 76-82
  • Section: SCIENTTIFIC RESEARCHES
  • URL: https://www.vestnikdv.ru/jour/article/view/164
  • DOI: https://doi.org/10.25208/0042-4609-2015-91-4-76-82

Abstract


Pemphigus vulgaris is a chronic autoimmune bullous disease characterized by the formation of blisters on the skin and/ or mucous tunics as a result of acantholysis. To search for new molecular and biological targets, study pathogenetic mechanisms of the disease development and develop new methods of treatment, it is urgent to create an experimental model of pemphigus in laboratory animals reproducing clinical, histological and immunological signs of pemphigus. Goal of the study. To develop an experimental model of pemphigus by injecting IgG produced from the blood serum taken from patients with pemphigus to neonatal mice of the BALB/c inbred line. Results. Accumulated IgG products taken from patients with pemphigus (main groups) and healthy volunteers (control group) were injected intraperitoneally to neonatal mice of the BALB/с in the doses of 10-30 mg per mouse. Clinical, histological and immunomorphological signs of pemphigus were revealed in the mice from the main group, which received intraperitoneal injections of IgG taken from patients with pemphigus in the dose of 30 mg per mouse. No signs of pemphigus were observed in the mice from the control group, which received injections of IgG taken from healthy people. This study confirms the role of pemphigus autoantibodies in the pathogenesis of pemphigus vulgaris and shows that passive transmission of antibodies to laboratory animals is possible.

A. A. Kubanov

State Research Center of Dermatovenereology and Cosmetology, Ministry of Healthcare of the Russian Federation

Author for correspondence.
Email: noemail@neicon.ru

Russian Federation

A. E. Karamova

State Research Center of Dermatovenereology and Cosmetology, Ministry of Healthcare of the Russian Federation

Email: noemail@neicon.ru

Russian Federation

K. V. Rog

State Research Center of Dermatovenereology and Cosmetology, Ministry of Healthcare of the Russian Federation

Email: noemail@neicon.ru

Russian Federation

T. V. Abramova

Russian Medical Academy of Postgraduate Studies, Ministry of Health of the Russian Federation

Email: abtava@mail.ru

Russian Federation

V. A. Smolyannikova

I. M. Sechenov First Moscow State Medical University

Email: noemail@neicon.ru

Russian Federation

A. N. Murashev

Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: noemail@neicon.ru

Russian Federation

D. A. Bondarenko

Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: noemail@neicon.ru

Russian Federation

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