Clinical and morphological diagnostics of skin manifestations in case of graft-versus-host disease

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Abstract

The graft-versus-host disease is a life-threatening complication of allogeneic bone marrow transplantation, which demands fast and reliable diagnostics. Goal. To study clinical features of the graft-versus-host disease as well as differential and diagnostic value of histological and immunohistochemical signs in skin biopsy samples in case of graft-versus-host disease (GvHD). Materials and methods. The authors made a comparative assessment of clinical data available for patients suffering from acute GvHD (50 patients) and toxic dermatitis (10 patients). Skin biopsy samples taken from patients from both groups underwent postmortem and immunohistochemistry examinations: the drugs were stained with hematoxylin and eosin as well as by using anti-CD3, CD20, CD4, CD8, FOXP3, CD56, CD1a and CD 68 antibodies. Results. Based on the results of clinical data assessments, no significant differences in the groups were revealed. The morphological examination revealed that the number of eosinophils, CD8+, CD1a+, CD3+ and FOXP3+ cells and share of FOXP3+ cells among Т lymphocytes was higher in the group with toxic dermatitis. Conclusion. An integrated assessment of clinical data and morphological examination results accompanied by the analysis of the population composition of the infiltrate is needed to diagnose the acute GvHD.

About the authors

A. YU. Krivolapova

Saint Petersburg State Medical University

Author for correspondence.
Email: akrivolapova@gmail.com
Russian Federation

I. O. Smirnova

Saint Petersburg State University

Email: noemail@neicon.ru
Russian Federation

YE. V. Lisukova

Saint Petersburg State Medical University

Email: noemail@neicon.ru
Russian Federation

I. S. Moiseyev

Saint Petersburg State Medical University

Email: noemail@neicon.ru
Russian Federation

I. E. Belousova

Military Medical Academy named after S.M. Kirov Ministry of Defense of the Russian Federation

Email: noemail@neicon.ru
Russian Federation

V. V. Baikov

Saint Petersburg State Medical University

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2015 Krivolapova A.Y., Smirnova I.O., Lisukova Y.V., Moiseyev I.S., Belousova I.E., Baikov V.V.

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