Antiangiogenic potential of beta-blockers in the context of juvenile hemangioma treatment

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Abstract


Juvenile hemangiomas (JH) — the most common tumor of childhood, which is estimated by various investigators found in 3–10 % of newborns resulting from the local development of significant violations of neoangiogenesis regulation. Research objective: determination of comparative antiangiogenic effectiveness and influence of beta-blockers on the level of a vascular endothelial factor of growth in an experiment. Material and methods. For determination of antiangiogenic effect of beta blockers, comparative studying of their influence on the level of a vascular endothelial factor of growth in an experiment on 72 nonlinear laboratory rats, by average weight 180 ± 20 g which were conditionally divided into 6 groups is executed: 1 — control, 2 — negative control — experimental ischemia (EI, crossing of femoral vessels), 3 — positive control (EI with bevacizumab introduction), 4 — EI with introduction of a timolol, 5 — EI with introduction of a betaksolol, 6 — EI with introduction of interferon alpha 2b. Results. The VEGF levels were: in 1 group — 1.50 ± 0.3 pg/ml, in 2 — 20.3 ± 3.2 pg/ml, 3 — 5.8 ± 0.9 pg/ml, 4 — 13.8 ± 1.4 pg/ml, 5 — 19.2 ± 2.3 pg/ml and 6 — 11.1 ± 2.2 pg/ml. Results of microscopy and immunohistochemical research demonstrate lack of activation of processes of neoangiogenesis in 1 group of animals. At animals of 2nd and 5th groups along with the expressed inflammatory processes the neoangiogenesis phenomena are established. Conclusion. Beta-blockers show the direct or mediated negative impact on synthesis of VEGF and oppression of neoangiogenesis. The activity of selective beta-blocker concerning neoangiogenesis suppression — was lower in comparison by activity non-selective that allows to consider the Timolol effective antiangiogenic remedy.


About the authors

Vl. V. Dubensky

Tver State Medical University, Ministry of Health of the Russian Federation

Author for correspondence.
Email: tgma.estet@yandex.ru

Russian Federation

Vladislav V. Dubensky — Cand. Sci. (Med.), Assoc. Prof., Prof. of the Department of Dermatovenereology with a Course of Cosmetology

tel.: +7 (961) 016-00-00

V. V. Dubensky

Tver State Medical University, Ministry of Health of the Russian Federation

Email: fake@neicon.ru

Russian Federation Valery V. Dubensky — Dr. Sci. (Med.), Prof., Head of the Department of Dermatovenereology with a Course of Cosmetology

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