Molecular-biological Methods of Research in Laboratory Diagnostics of Leprosy: Epidemiological Analysis, Genetic Determinants of Resistance to Antimicrobial Drugs

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Abstract


The review of literature presents analysis of the current state of molecular biological methods for research of Mycobacterium leprae. The characteristics and feasibility of application of the methods in use for diagnostics and epidemiological monitoring of the leprosy pathogen are discussed, as well as identification of possible genetic determinants of antibiotic resistance. The present knowledge is provided of genetic characteristics of mycobacteria of the above type, which enable to lay the foundations for development of methods for leprosy diagnostics, as well as also for their significant improvement.

About the authors

O. A. Obraztsova

State Research Center of Dermatovenereology and Cosmetology, Ministry of Health of the Russian Federation

Author for correspondence.
Email: valeeva19@gmail.com

Russian Federation

PhD (biology), Senior Scientific Researcher, Department of Laboratory Diagnostics of STIs and Dermatosis, State Research Center of Dermatovenereology and Cosmetology, Ministry of Health of the Russian Federation

References

  1. Скрипкин Ю. К., Кубанова А. А., Акимов В. Г. Кожные и венерические болезни. М.: ГЭОТАР-Медиа, 2009. [Skripkin Yu. K., Kubanova A. A, Akimov V. G. Dermal and Venereal Diseases. M.: GEOTAR-Media, 2009.]
  2. Дегтярев О. В., Иншина Е. А., Метревели Г. В., Янчевская Е. Ю. Рецидивы лепры. Астраханский медицинский журнал. 2015;10:6–14. [Degtyarev O. V., Inshina E. A., Metreveli G. V., Yanchevskaya E. Yu. The Relapse of Leprosy. Astrakhan Medical Journal. 2015;10:6–14.]
  3. WHA44.9: Adoption of Multidrug Therapy for Elimination of Leprosy as a Public Health Problem. 1991.
  4. Лепра. Доклад ВОЗ ЕВ 126/41. Пункт 4.21. Сто двадцать шестая сессия ВОЗ, 2010. [Leprosy. WHO Report EB 126/41. P. 4.21. 126th session of WHO, 2010.]
  5. Aftab H., Nielsen S. D., Bygbjerg C. Leprosy in Denmark 1980–2010: a Review of 15 Cases. BMC Res. Notes. 2016;9(1):1–9.
  6. Bret S., Flageul B., Girault P. Y. et al. Epidemiological Survey of Leprosy Conducted in Metropolitan France between 2009 and 2010. Ann. Dermatol. Venereol. 2013;140(5):347–352.
  7. Дуйко В. В. Особенности организации противолепрозных мероприятий в России на современном этапе. Проблемы социальной гигиены и история медицины. 2013;(2):31–32. [Duyko V. V. Features of the Organization of Anti-Leprosy Measures in Russia at the Present Stage. Problems of Social Hygiene and the History of Medicine. 2013;(2):31–32.]
  8. Дуйко В. В. О противолепрозных мероприятиях в России. Бюллетень Национального научно-исследовательского института общественного здоровья имени Н. А. Семашко. 2013;(1):154–157. [Duyko V. V. About Anti-Leprosy Measures in Russia. Bulletin of the N.A. Semashko National Research Institute of Public Health. 2013;(1):154–157.]
  9. Ющенко A. A., Урляпова Н. Г., Дуйко В. В. Анализ и прогноз эпи-демиологической ситуации по лепре в России. Астрахань, 2002. C. 43. (Yushchenko A. A., Urlyapova N. G., Duyko V. V. Analysis and Forecast of the Leprosy Epidemiological Situation in Russia. Astrakhan, 2002. P. 43.)
  10. Ridley D. S., Jopling W. H. Classification of Leprosy According to Immunity. A Five-Group System. Int. J. Lepr. Other Mycobact. Dis. 1966;34(3):255–273.
  11. Приказ Минздрава СССР от 14.12.1990 № 483 «Об утверждении инструкций по борьбе с лепрой». [The Order of USSR Ministry of Health of 14.12.1990 N 483 “On Approving Instructions for the Fight Against Leprosy”.]
  12. Скрипкин Ю. К., Бутов Ю. С., Иванов О. Л. Дерматовенерология. Национальное руководство. М.: ГЭОТАР-Медиа, 2013. [Skripkin Yu. K., Butov Yu. S., Ivanov O. L. Dermatovenereology. National Leadership. M.: GEOTAR-Media, 2013.]
  13. Cole S. T., Eiglmeier K., Parkhill J. et al. Massive Gene Decay in the Leprosy Bacillus. Nature. 2001;409(6823):1007–1011.
  14. Nunzi E., Massone C. Leprosy. A practical guide. Springer, 2015.
  15. Lavania M., Jadhav R., Turankar R. P. et al. Genotyping of Mycobacterium leprae Strains from a Region of High Endemic Leprosy Prevalence in India. Infect. Genet. Evol. 2015;36:256–261.
  16. Turankar R. P., Pandey S., Lavania M. et al. Comparative Evaluation of PCR Amplification of RLEP, 16S rRNA, rpoT and Sod A Gene Targets for Detection of M. leprae DNA from Clinical and Environmental Samples. Int. J. Mycobacteriol. 2015;4(1):54–59.
  17. Memon R. A., Hussain R., Raynes J. G. et al. Alterations in Serum Lipids in Lepromatous Leprosy Patients with and without ENL Reactions and Their Relationship to Acute Phase Proteins. Int. J. Lepr. Other Mycobact. Dis. 1996;64(2):115–122.
  18. Martinez A. N., Ribeiro-Alves M., Sarno E. N., Moraes M. O. Evaluation of qPCR-Based Assays for Leprosy Diagnosis Directly in Clinical Specimens. PLoS Negl. Trop. Dis. 2011;5(10):1–8.
  19. Yan W., Xing Y , Yuan L. C. et al. Application of RLEP Real-Time PCR for Detection of M. leprae DNA in Paraffin-Embedded Skin Biopsy Specimens for Diagnosis of Paucibacillary Leprosy. Am. J. Trop. Med. Hyg. 2014;90(3):524–529.
  20. Yoon K. H., Cho S. N., Lee M. K. et al. Evaluation of Polymerase Chain Reaction Amplification of Mycobacterium leprae Specific Repetitive Sequence in Biopsy Specimens from Leprosy Patients. J. Clin. Microbiol. 1993;31(4):895–899.
  21. Monot M., Honore´ N., Garnier T. et al. On the Origin of Leprosy. Science. 2005;308(5724):1040–1042.
  22. Monot M., Honore´ N., Garnier T. et al. Comparative Genomic and Phylogeographic Analysis of Mycobacterium leprae. Nat. Genet. 2009;41(12):1282–1289.
  23. Lima L., Fontes A., Li W. et al. Intrapatient Comparison of Mycobacterium leprae by VNTR Analysis in Nasal Secretions and Skin Biopsy in a Brazilian Leprosy Endemic Region. Lepr. Rev. 2016;87(4):486–500.
  24. Monot M., Honore´ N., Balie`re C. et al. Are Variable-Number Tandem Repeats Appropriate for Genotyping Mycobacterium leprae? J. Clin. Microbiol. 2008;46(7):2291–2297.
  25. Chemotherapy of Leprosy for Control Programmes. Technical Report Series 675. Geneva: World Health Organization, 1982.
  26. Guidelines for Global Surveillance of Drug Resistance in Leprosy. World Health Organization, 2009.
  27. Matsuoka M., Aye K. S., Kyaw K. et al. A Novel Method for Simple Detection of Mutations Conferring Drug Resistance in Mycobacterium leprae, Based on a DNA Microarray, and its Applicability in Developing Countries. J. Med. Microbiol. 2008;57(Pt 10):1213–1219.
  28. Ling D. I., Zwerling A. A., Pai M. GenoType MTBDR Assays for the Diagnosis of Multidrug-Resistant Tuberculosis: a Meta-Analysis. Eur. Respir. J. 2008;32(5):1165–1174.

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